Authors:Roya Khosravi-Far, Zahra Zakeri, Richard A. Lockshin
Publisher: Academic Press
Keywords: methods, enzymology, volume, part, cell, death, programmed
Number of Pages: 544
Published: 2008-09-11
List price: $182.00
ISBN-10: 0123743125
ISBN-13: 9780123743121

The 2002 Nobel Prize in Physiology or Medicine was awarded to Sydney Brenner (United Kingdom), H. Robert Horvitz (US) and John E. Sulston (UK) "for their discoveries concerning genetic regulation of organ development and programmed cell death." Cell death is a fundamental aspect of embryonic development, normal cellular turnover and maintenance of homeostasis (maintaining a stable, constant environment) on the one hand, and aging and disease on the other. This volume addresses the significant advances with the techniques that are being used to analyze cell death.

Authors:John N. Abelson, Melvin I. Simon, Harry Walter, Göte
Publisher: Academic Press
Keywords: methods, enzymology, volume, systems, phase, aqueous
Number of Pages: 725
Published: 1994-05-02
List price: $182.00
ISBN-10: 0121821293
ISBN-13: 9780121821296

This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features* Use of affinity partitioning for enzyme purification* Separation and study of cells* Isolation of plasma membranes* Large scale biotechnical use

Authors:William E. Balch, Channing J. Der, Alan Hall,
Publisher: Academic Press
Keywords: gtpases, ras, family, small, effectors, enzymology, volume, regulators, methods
Number of Pages: 780
Published: 2006-04-21
List price: $182.00
ISBN-10: 0121828123
ISBN-13: 9780121828127

The Ras superfamily (>150 human members) encompasses Ras GTPases involved in cell proliferation, Rho GTPases involved in regulating the cytoskeleton, Rab GTPases involved in membrane targeting/fusion and a group of GTPases including Sar1, Arf, Arl and dynamin involved in vesicle budding/fission. These GTPases act as molecular switches and their activities are controlled by a large number of regulatory molecules that affect either GTP loading (guanine nucleotide exchange factors or GEFs) or GTP hydrolysis (GTPase activating proteins or GAPs). In their active state, they interact with a continua

Authors:John N. Abelson, Melvin I. Simon, Bernardo Rudy, Lind
Publisher: Academic Press
Keywords: vol, paper, reprint, enzymology, methods, channels, volume, ion
Number of Pages: 917
Published: 1997-10-02
List price: $109.00
ISBN-10: 012601695X
ISBN-13: 9780126016956

This is an unabridged, comb bound version of Ion Channels: Volume 207 of Methods of Enzymology. Techniques for the electrophysiological recording of ion channels in a number of different cell types, including microorganisms, are described in this volume. Key Features* Applications of electrophysiological techniques* Expression in heterologous systems* Reconstitution of lipid bilayers* Purification and characterization of ion channel proteins and genes* Recording of ion channels of intracellular membranes and microorganisms* Data storage and analysis

Authors:John N. Abelson, Melvin I. Simon, Helmut Sies,
Publisher: Academic Press
Keywords: volume, methods, enzymology, part, systems, radicals, biological, oxygen
Number of Pages: 711
Published: 1994-04-05
List price: $182.00
ISBN-10: 012182134X
ISBN-13: 9780121821340

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. Key Features* Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or

Authors:John N. Abelson, Melvin I. Simon, Suresh V. Ambudkar
Publisher: Academic Press
Keywords: volume, methods, enzymology, aspects, molecular, transporters, biochemical, cellular, abc
Number of Pages: 853
Published: 1998-08-14
List price: $182.00
ISBN-10: 0121821935
ISBN-13: 9780121821937

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchersin all fields of life sciences. Key Features* Prokaryotic ABC Transporters* Eukaryotic ABC Transporters*

Authors:Lynne E. Maquat, Megerditch Kiledjian,
Publisher: Academic Press
Keywords: decay, volume, methods, enzymology, mrna, analysis, turnover, eukaryotes, nucleases, pathways, rna
Number of Pages: 656
Published: 2008-12-29
List price: $182.00
ISBN-10: 0123743788
ISBN-13: 9780123743787

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. The co