Author: Charles W. Carter Jr.
Publisher: Academic Press
Keywords: methods, enzymology, volume, part, crystallography, macromolecular
Number of Pages: 679
Published: 2003-12-24
List price: $182.00
ISBN-10: 0121827771
ISBN-13: 9780121827779

Accurate molecular structures is vital for rational drug design and for structure based functional studies directed toward the development of effective therapeutic agents and drugs. Crystallography can reliably predict structure, both in terms of folding and atomic details of bonding. * Phases* Map interpretation and refinement* Analysis and software

Author: David A. Cheresh
Publisher: Academic Press
Keywords: methods, enzymology, volume, systems, vitro, angiogenesis
Number of Pages: 432
Published: 2008-09-16
List price: $182.00
ISBN-10: 012374315X
ISBN-13: 9780123743152

Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result when blood vessels either grow excessively or insufficiently. * Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition * Provides the "builder’s manual" for essential techniques--a one-stop shop tha

Authors:David R. Engelke, John J. Rossi,
Publisher: Academic Press
Keywords: interference, rna, volyme, enzymology, methods
Number of Pages: 496
Published: 2005-02-24
List price: $182.00
ISBN-10: 0121827976
ISBN-13: 9780121827977

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology r

Authors:Judith L. Campbell, Paul Modrich,
Publisher: Academic Press
Keywords: methods, enzymology, volume, part, repair, dna
Number of Pages: 616
Published: 2006-05-15
List price: $182.00
ISBN-10: 012182814X
ISBN-13: 9780121828141

This volume emphasizes the intracellular consequences of DNA damage, describing procedures for analysis of checkpoint responses, DNA repair in vivo, replication fork encounter of DNA damage, as well as biological methods for analysis of mutation production and chromosome rearrangements. It also describes molecular methods for analysis of a number of genome maintenance activities including DNA ligases, helicases, and single-strand binding proteins. *Part B of a 2-part series*Addresses DNA maintenance enzymes*Discusses damage signaling*Presents In vivo anal

Authors:Tom W. Muir, John N. Abelso,
Publisher: Academic Pre
Keywords: methods, enzymology, volume, acids, natural, amino, non
Number of Pages: 312
Published: 2009-08-28
List price: $182.00
ISBN-10: 0123743109
ISBN-13: 9780123743107

By combining the tools of organic chemistry with those of physical biochemistry and cell biology, ’Non-Natural Amino Acids’ aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. This volume brings together the tools and principles of chemistry with the molecules and processes of living cells to create molecules with new properties and functions found neither in nature nor in the test tube. By studying the structure and function of these resulting molecules, new insights can be gained into the molecular m

Author: A.L. Burlingame
Publisher: Academic Press
Keywords: modified, proteins, glycoconjugates, spectrometry, mass, enzymology, volume, methods
Number of Pages: 472
Published: 2005-12-27
List price: $182.00
ISBN-10: 0121828107
ISBN-13: 9780121828103

This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages

Authors:Ludwig Brand, Michael L. Johnson,
Publisher: Academic Press
Keywords: enzymology, methods, volume, spectroscopy, fluorescence
Number of Pages: 400
Published: 2008-12-25
List price: $182.00
ISBN-10: 0123745861
ISBN-13: 9780123745866

Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating tec