Guide to Yeast Genetics and Molecular Biology, Part A, Volume 194 (Methods in Enzymology Series, 194)

Book Description:

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast.
* Basic Methods in Yeast Genetics
* Physical and genetic mapping
* Making and recovering mutants
* Cloning and Recombinant DNA Methods
* High-efficiency transformation
* Preparation of yeast artificial chromosome vectors
* Basic Methods of Cell Biology
* Immunomicroscopy
* Protein targeting assays
* Biochemistry of Gene Expression
* Vectors for regulated expression
* Isolation of labeled and unlabeled DNA, RNA, and protein